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The Efficient Termination of ADA Tasks in a Multiprocessor.


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Corresponding authors. Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Lehrstuhl Biochemie III, 93053 Regensburg, Germany. Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2472; Fax:+49 941 943 2474; E-mail: [email protected]

Corresponding authors. Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Lehrstuhl Biochemie III, 93053 Regensburg, Germany. Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2472; Fax:+49 941 943 2474; E-mail: [email protected]

Corresponding authors. Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Lehrstuhl Biochemie III, 93053 Regensburg, Germany. Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2472; Fax:+49 941 943 2474; E-mail: [email protected]

Transcription termination requires specific DNA and/or RNA elements that directly or indirectly interfere with the elongating RNA polymerase machinery. Properly terminated transcription results in a length restricted transcript, which is disposed for posttranscriptional modifications and the release of the RNA polymerase from the DNA template. Similarities in the structure of bacterial, eukaryotic and archaeal ternary transcription complexes indicate that termination mechanisms must overcome related energetic and structural constraints ( Peters et al , 2011 ).

In contrast, cleavage by the Reb1–MNase fusion protein was (if at all) barely detectable at the integrated terminator element ( Figure 2B , Reb1–MNase), whereas robust cleavage of the fusion protein was observed at the Pol I promoter-proximal Reb1-binding site in both, the strain carrying a terminator element or no insertion in ITS1 ( Figure 2B , Reb1–MNase, asterisk on the left).

Altogether, these data suggest that Ydr026c binding to the rDNA terminator element is required to efficiently terminate transcript elongation and Pol I–rDNA association.

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Corresponding authors. Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Lehrstuhl Biochemie III, 93053 Regensburg, Germany. Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2472; Fax:+49 941 943 2474; E-mail: [email protected]

Corresponding authors. Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Lehrstuhl Biochemie III, 93053 Regensburg, Germany. Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2472; Fax:+49 941 943 2474; E-mail: [email protected]

Corresponding authors. Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Lehrstuhl Biochemie III, 93053 Regensburg, Germany. Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2846; Fax:+49 941 943 2474; E-mail: [email protected] or Tel.:+49 941 943 2472; Fax:+49 941 943 2474; E-mail: [email protected]

Transcription termination requires specific DNA and/or RNA elements that directly or indirectly interfere with the elongating RNA polymerase machinery. Properly terminated transcription results in a length restricted transcript, which is disposed for posttranscriptional modifications and the release of the RNA polymerase from the DNA template. Similarities in the structure of bacterial, eukaryotic and archaeal ternary transcription complexes indicate that termination mechanisms must overcome related energetic and structural constraints ( Peters et al , 2011 ).

In contrast, cleavage by the Reb1–MNase fusion protein was (if at all) barely detectable at the integrated terminator element ( Figure 2B , Reb1–MNase), whereas robust cleavage of the fusion protein was observed at the Pol I promoter-proximal Reb1-binding site in both, the strain carrying a terminator element or no insertion in ITS1 ( Figure 2B , Reb1–MNase, asterisk on the left).

Altogether, these data suggest that Ydr026c binding to the rDNA terminator element is required to efficiently terminate transcript elongation and Pol I–rDNA association.

Recently I wrote about things you should never say when you fire an employee . A few people emailed to say, in essence, "Great, but what should I say and do when I fire an employee?

Here's how to make a bad situation better—or at least as "better" as it can possibly be—when you have to fire an employee for cause:

Seems obvious, right? Not always: The heat of the moment can cause you to make a snap decision that is neither correct nor fair.

Even if you have a zero-tolerance policy for certain behaviors, take a few minutes to make sure the employee's action truly falls within the parameters of that policy. When you're mad (or really disappointed) it's easy to think, "That's it... she has to go," and unintentionally forget about guidelines and precedents. While you can bring an employee back on after you make a mistake, no one will ever forget what happened.

Except where zero-tolerance policy violations are concerned, firing an employee should always be the last step in a relatively formal and structured process: Identify sub-par performance, provide additional training or resources, set targets and time lines for performance improvement, follow up when progress is lacking—and document each step in writing.

Documentation not only protects your business, it also helps ensure the employee was given every chance to succeed. You—and the employee—deserve more than a trail of bread crumbs.

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